Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Anacardic Acid (6-pentadecylsalicylic Acid) 是从腰果壳中提取液中分离到的酸类物质,是一种组蛋白乙酰转移酶抑制剂,对 p300 和 PCAF 的 IC50值分别为 ∼8.5 μM 和 ∼5 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 381 | 现货 | ||
10 mg | ¥ 597 | 现货 | ||
25 mg | ¥ 1,227 | 现货 | ||
50 mg | ¥ 1,990 | 现货 | ||
100 mg | ¥ 2,947 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 381 | 现货 |
产品描述 | Anacardic Acid (6-pentadecylsalicylic Acid) is an effective inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases. It also has antimicrobial activity, antibacterial activity, and inhibits prostaglandin synthase, lipoxygenase, and tyrosinase. |
靶点活性 | PCAF(p300/CREB-binding protein-associated factor):5 μM, p300 HAT:8.5 μM |
体外活性 | Anacardic acid is an inhibitor of several Clinicalally targeted enzymes such as HATs, NF-kB, LOX-1, xanthine oxidase, tyrosinase, and ureases. Anacardic acid is also an effective inhibitor of Tip60 in vitro. It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells and sensitizes the cells to the cytotoxic effects of radiation. |
体内活性 | Anacardic acid decreases over-expression of Gata4, α-MHC, and cTnT in fetal mouse hearts exposed to ethanol. Anacardic acid inhibits binding of PCAF, P300 to the promoter of Gata4. |
激酶实验 | HAT and HDAC Activity Assays :After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits. |
细胞实验 | HeLa and 293T cells are grown in Dulbecco's modified Eagle's medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum. For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid (0-100 μM) for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days. |
动物实验 | Animal Models: 9 to 11 week old Kunming mice. Formulation: Dissolved in DMSO,co-injected with ethanol. Dosages: 0,1.25,2.5,5,10 mg/kg. Administration: i.p. |
别名 | 漆树酸, 6-pentadecylsalicylic Acid, Hydroginkgolic acid |
分子量 | 348.52 |
分子式 | C22H36O3 |
CAS No. | 16611-84-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 17.4 mg/mL (50 mM)
DMSO: 15 mg/mL (43.04 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.8693 mL | 14.3464 mL | 28.6928 mL | 71.7319 mL |
5 mM | 0.5739 mL | 2.8693 mL | 5.7386 mL | 14.3464 mL | |
10 mM | 0.2869 mL | 1.4346 mL | 2.8693 mL | 7.1732 mL | |
20 mM | 0.1435 mL | 0.7173 mL | 1.4346 mL | 3.5866 mL | |
Ethanol | 50 mM | 0.0574 mL | 0.2869 mL | 0.5739 mL | 1.4346 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
白果叶 | Ginkgo bilobe L. | 平 | 苦, 甘, 涩 | 心, 肺, 脾 |
都咸子 | Anacardium occidentale L. | 平 | 甘 | 肺 |
方剂名称 | 处方组成 | 剂型 | 处方来源 |
白果叶散 | 珍珠,轻粉,雄黄,白果叶 | 散剂 | 《疡医大全》卷十八引(吴氏家秘》。 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Anacardic Acid 16611-84-0 Chromatin/Epigenetic Microbiology/Virology Others Histone Acetyltransferase Antibacterial Epigenetic Reader Domain Bacterial HATs 漆树酸 6-pentadecylsalicylic Acid inhibit HAT Ginkgolic Acid C15:0 Inhibitor Hydroginkgolic acid inhibitor